Now you must mix this solution once a day. If you put tin foil in the holes, take it out and place micropore tape over the hole.įor Multispore Syringe or Mushroom Culture Syringe: shake very well, (optional – flame sterilize needle), clean needle with alcohol wipe, lift up micropore tape, and inject 1-2cc of solution per 500mL.įor Fruit Body, Spore Print, or Agar Culture: Take piece of culture/fruit/pile of spores and simply dump them into the jar. Remove jars from pressure cooker, place them in the sterile area, remove tin foil from jars and wipe lids with alcohol wipes. Ideally setup and sterilize your still air box or flow hood, otherwise sterilize the area of inoculation by wiping it down with alcohol. Let them cool to at least room temperature before the next step. Place the jars into your pressure cooker and sterilize them at 15psi for 35 to 40 minutes. Then after you do one or the other, cover each jar lid with tinfoil.

Simply fill each jar with water and the corresponding amount of LME and shake it up well (this is where the unpoked lid comes in).Īgain, you have 2 options here – you can fill the holes in the jar lids with tin foil (make sure it fits snug) or you can use micropore tape. You can calculate how much water and LME (or honey) you need and put that in a pot to heat on low for 5-10 minutes or 2. Simply make sure your jars and jar lids are cleaned very well, and then poke 1 hole in the lid of each jar with a screwdriver or syringes (tip – keep one extra lid unpoked for the next step).

You can use for the most part any size jar. You can inoculate this nutrient solution with either a multispore syringe or spore print, mushroom fruiting body, or another mushroom liquid culture. Our favorite LC recipe is a Light Malt Extract (LME) liquid culture recipe – but you can simply replace the LME in our recipe with 24mL honey if you wish.Įssentially the liquid culture recipe consists simply of 1g of LME (or 24mL of honey) per 600mL of water. If you find yourself wondering “why do these spores take so long to grow?”, spores to liquid culture is certain to fix those problems! Making liquid culture is a process that utilizes mushroom spores, mushroom fruit body, or another mycelium liquid culture to create a powerful and convenient solution used for growing mushrooms.

Mushroom spore syringe use